Journal: Materials Today Bio

Article Title: Replenishment of mitochondrial Na + and H + by ionophores potentiates cutaneous wound healing in diabetes

doi: 10.1016/j.mtbio.2024.101056

Figure Lengend Snippet: Monensin An up-regulates mitochondrially encoded genes. (A) Overview of the monensin A mediated alteration of gene expression on HACAT cells using bulk RNA-seq. (B) Pathway enrichment analysis arranging the top up-regulated pathways according to the adjusted p-value (left). The altered information on the top-ranking pathway was further excavated (right). (C) Heatmap shows monensin A-triggered actively expressed genes involved in the top-ranking pathway. Data are color-coded to reflect the relative expression level of mitochondrially encoded genes. All data are normalized to their matched control. (D) PCR-based validation of the mRNA expression of mitochondrial genes in HACAT cells treated with or without monensin A (125 nM) for 3 h. Data are represented as mean ± SD. Two-tailed Student's unpaired t -test (D). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: RNA samples were submitted to LC Sciences (Hangzhou, China) for bulk RNA sequencing using the Illumina platform.

Techniques: Gene Expression, RNA Sequencing, Expressing, Control, Biomarker Discovery, Two Tailed Test

Journal: Cell Death & Disease

Article Title: The TRPM7 channel reprograms cellular glycolysis to drive tumorigenesis and angiogenesis

doi: 10.1038/s41419-023-05701-7

Figure Lengend Snippet: A Volcano plots of glucose metabolism-related genes that are induced in WT versus TRPM7KO cells. Genes with a fold-change ≥2 and P value of < 0.05 are shown. B RT-qPCR analysis validating the decreased expression of glucose catabolic genes from RNA-seq in TRPM7KO cells. C Immunofluorescent staining of TRPM7, SLC2A3 and DAPI in the xenograft tumor (scale bars, 100 μm). D Immunohistochemical staining for TRPM7, SLC2A1 and SLC2A3 in tumor tissue (scale bars, 100 μm). E , F Increased SLC2A3 in naltriben-induced (25 μM, 24 h) WT cells but not TRPM7KO cells. Quantification of protein was below. Two groups of samples were compared by unpaired two-tailed Student’s t -test. Multiple groups were compared by ANOVA.

Article Snippet: The bulk RNA sequencing was performed by Metware Co., LTD.

Techniques: Quantitative RT-PCR, Expressing, RNA Sequencing, Staining, Immunohistochemical staining, Two Tailed Test

Journal: Osteoarthritis and Cartilage Open

Article Title: Transcriptomics and metabolomics: Challenges of studying obesity in osteoarthritis

doi: 10.1016/j.ocarto.2024.100479

Figure Lengend Snippet: Summary of 15 studies identified investigating obesity as a variable related to human or animal model osteoarthritis using transcriptomics or metabolomics. Publications with associated Gene Expression Omnibus dataset accession numbers (GSE) are indicated.

Article Snippet: Schott et al. (2018) [ ] (GSE98287) , Mouse diet-induced obesity , • Low-fat diet-fed mice • high-fat diet-fed mice , Not Reported , Transcriptomics , Bulk RNA sequencing , Colon.

Techniques: Animal Model, Gene Expression, Comparison, RNA Sequencing, Mouse Assay, Microarray, Clinical Proteomics, Structural Proteomics, Control, Gas Chromatography